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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:20152*  Cite 
Description

This is the original movie of a Balamuthia mandrillaris ameba within a culture of monkey kidney cells in the background. The comparatively thick body of the ameba has a number of pseudopodia extending from it and a large prominent nucleus. The action of the pseudopodia is important in the feeding of the amebas. Their tips have a pointed, sharp, dense extension that has been seen to grow out, and move together with a snap (about 1 second). This action is a typical movement of the tips of the pseudopodia. Accompanying photographs are enlargements of the tips of pseudopodia to demonstrate their flexibility and movement, and a second set of photographs that demonstrate the puncture of the plasma membrane of a rounded, clear monkey kidney cell with a large, rounded, clear nucleus with an elongated dense nucleolus. The first shows an indentation in the cell membrane, the withdrawal of the pseudopodia tip and the subsequent rupture of the membrane in the area indented by the amebic tip. The eventual break in the membrane may have resulted from the release of a proteolytic enzyme. Adapted with permission. Details of preparation can be found to accompany CIL:19325.

Biological Sources
NCBI Organism Classification
Balamuthia mandrillaris
Cell Type
cell by organism
eukaryotic cell
Eukaryotic Protist
Amoeboid Protist
Cell Line
RP5
Cellular Component
cell
pseudopodium
Attribution
Names
Thelma Dunnebacke
Published
J. Euk. Microbiol. 2007. 54:452-464
Pubmed
17910691
Link
http://onlinelibrary.wiley.com/doi/10.1111/j....
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL20152
Archival Resource Key (ARK)
ark:/b7295/w9cil20152
Grouping This image is part of a group.
Imaging
Image Type
microscopy
in-focus phase contrast
Image Mode
retardance
phase contrast microscopy
Parameters Imaged
boundaries between regions with different refractive index
Source of Contrast
boundaries between regions with different refractive index
Visualization Methods
natural boundaries
Processing History
living tissue
recorded image
charge coupled device (CCD)
Data Qualifiers
raw, unprocessed data
Sample Preparation
Methods
living tissue
Methods
living tissue
whole mounted tissue
Dimensions
Spatial Axis Image Size Pixel Size
X 320px ——
Y 240px ——