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Description

Distribution of interneurons expressing EGFP from the GAD65 promoter in the day 14 mouse hippocampus colabelled for the CB1 cannabinoid receptor (red) and counterstained with DAPI (blue) to show the cell layers. EGFP expression was amplified with chicken anti-GFP (Abcam, 1:2000). GAD65-EGFP expressing neurons are enriched in the dentate gyrus and a band of EGFP is present in the inner dentate molecular layer. CB1 immunoreactivity (L15 rabbit polyclonal 1:200, K. Mackie) is prominent in the molecular layer but also present at lower levers in the outer portion of the dentate molecular layer. Diffuse CB1 immunoreactivity is also present in the hilus. Section (40 um) was mounted with Fluoromount G and imaged with a 10X, 0.3 NA objective on a Zeiss Axiovert microscope. AF568 was excited at 562/40 nm and emission collected at 624/40 nm with Semrock filters and a single band dichroic (530-585 nm reflected, 601-800 nm transmitted). AF488 was excited at 482/35 nm and emission collected at 536/40 nm with Semrock filters and a single band dichroic (446-499 nm reflected, 513-735 nm transmitted). DAPI was excited at 350/50 nm and emission collected at 460/50 nm with a CLP400 dichroic (Chroma). Single images were captured for each channel using a Zeiss Axiocam MR CCD and pseudo-colored using Axiovision. Linear background subtraction was used on the presented image.

Biological Sources

NCBI Organism Classification
Mus musculus
(house mouse)
Cell Type
CNS neuron (sensu Vertebrata)
interneuron
hippocampal granule cell
Cellular Component
neuronal cell body membrane
axon
nucleus

Biological Context

Biological Process
cell communication by chemical coupling
Molecular Function
cannabinoid receptor activity

Attribution

Name
Margaret I. Davis
*CIL – Cell Image Library accession number. Please use this to reference an image.