Licensing

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Description

Untreated 16-cell purple urchin embryo with EMTB-3G (microtubules) in shown cyan and mC-H2B (chromatin) in yellow.). The video shows normal correlation between aster development, furrowing, and the extent of separation of sister chromatids. Embryos were imaged with a laser scanning confocal microscope (Radiance 2000; Bio-Rad Laboratories) mounted on a compound microscope (E800; Nikon) using a 40× 1.3 NA Plan-Fluor oil lens. Room temperature was maintained at 14â��17°C. The video corresponds to the first segment of video 5 and Fig. S3A in J Cell Biol. 2009 Dec 14;187(6):831-45. Note this video is the control video for an embryo treated with trichostatin A (TSA), an inhibitor of HDAC6 (a tubulin deacetylase) that selectively disrupts dynamic microtubules in mammalian cells that is also part of this image group.

Biological Sources

NCBI Organism Classification
Strongylocentrotus purpuratus
(purple urchin)
Cell Line
Embryo
Cellular Component
microtubule
ensconsin
aster
cleavage furrow
histone

Biological Context

Biological Process
cytokinesis after mitosis

Attribution

Names
George von Dassow
Koen J.C. Verbrugghe
Ann L. Miller
Jenny R. Sider
William M. Bement
Published
J Cell Biol. 2009 Dec 14;187(6):831-45.
Pubmed
20008563

Grouping

This image is part of a group.

Imaging

Image Type
recorded image
Imaging Mode
single-spot confocal microscopy
Parameters Imaged
fluorescence emission
Source of Contrast
distribution of a specific protein
Visualization Methods
EGFP
mCherryFP
Processing History
unprocessed raw data

Sample Preparation

Methods
living tissue
Relation To Intact Cell
whole mounted tissue

Dimensions

Spatial Axis Image Size Pixel Size
X 420px 0.25μm
Y 420px 0.25μm
Time 12 sec 131
*CIL – Cell Image Library accession number. Please use this to reference an image.