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Description

Microtubules in live urchin embryos expressing three tandem GFPs fused to the ensconsin microtubule-binding domain (EMTB-3G; Faire et al., 1999). Microtubules approach the cortex everywhere before anaphase onset (1 min, 30 s). During anaphase, just before furrowing, many astral microtubules penetrate both polar and equatorial cortex. Embryos were imaged with a laser scanning confocal microscope (Radiance 2000; Bio-Rad Laboratories) mounted on a compound microscope (E800; Nikon) using a 40× 1.3 NA Plan-Fluor oil lens. Room temperature was maintained at 14塙°C, which is tolerated well. Related to Figure 1 and video 1 of J Cell Biol. 2009 Dec 14;187(6):831-45.

Biological Sources

NCBI Organism Classification
Strongylocentrotus purpuratus
(purple urchin)
Cell Line
Embryo
Cellular Component
microtubule
ensconsin
astral microtubule
cell cortex
cleavage furrow

Biological Context

Biological Process
mitotic anaphase
cytokinesis after mitosis

Attribution

Names
George von Dassow
Koen J.C. Verbrugghe
Ann L. Miller
Jenny R. Sider
William M. Bement
Published
J Cell Biol. 2009 Dec 14;187(6):831-45.
Pubmed
20008563

Grouping

This image is part of a group.

Imaging

Image Type
recorded image
Imaging Mode
single-spot confocal microscopy
Parameters Imaged
fluorescence emission
Source of Contrast
distribution of a specific protein
Visualization Methods
EGFP
Processing History
unprocessed raw data

Sample Preparation

Methods
living tissue
Relation To Intact Cell
whole mounted tissue

Dimensions

Spatial Axis Image Size Pixel Size
X 512px .2μm
Y 512px .2μm
Time 10 sec 101
*CIL – Cell Image Library accession number. Please use this to reference an image.