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Description

The intracellular mobility of MeCP2 (a methylated DNA-binding protein) followed in a mouse fibroblast stably expressing GFP-MeCP2 through a time series of images captured by confocal microscopy after bleaching a 2 micrometer circular area of euchromatin (center, right). Fluorescence recovery after photobleaching (FRAP) is rapid, indicating that MeCP2 in euchromatin is highly mobile.

Technical Details

Images were obtained using a Zeiss 510 Meta confocal microscope with excitation at 488 nm and emission between 505 nm and 530 nm. An initial scan of the cell was followed by the bleaching of a 2 um diameter spot of euchromatin, and 60 successive images recorded at 5 s intervals to measure fluorescence recovery due to MeCP2 mobility.

Biological Sources

NCBI Organism Classification
Mus musculus
(house mouse)
Cell Type
fibroblast
Cellular Component
nucleus
euchromatin
nuclear chromatin

Biological Context

Biological Process
nucleus organization
chromatin organization

Attribution

Names
rajarshi ghosh
,/chris woodcock

Imaging

Image Type
recorded image
Imaging Mode
single-spot confocal microscopy
Parameters Imaged
fluorescence emission
Source of Contrast
fluorescence emission
Visualization Methods
fluorescent proteins derived from Aequorea victoria
Processing History
unprocessed raw data

Sample Preparation

Methods
whole mounted tissue
Relation To Intact Cell
living tissue

Dimensions

Spatial Axis Image Size Pixel Size
X 512px 0.04μm
Y 512px 0.04μm
Time 5 sec 61
*CIL – Cell Image Library accession number. Please use this to reference an image.