Alternate header for print version


Licensing
This image is copyright protected. Any public or private use of this image is subject to prevailing copyright laws. Please contact the content provider of this image for permission requests.
tweet  
Comments: Powered by Pnyxe
*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:13007*  Cite 
Description

Section from rat brain cortex showing a subset of fluorescent-protein expressing neurons and microglia. Most prominent in this image are the cell bodies and proximal dendrites (including the stalk of the apical dendrite, oriented toward the lower left corner) of two pyramidal neurons (one red, and one green), and fine axons and dendrites from many other neurons coursing through the tissue. The colors represent the Z-position in a maximum intensity projection of an image (compiled from 90 image planes, 0.1µm intervals), and were generated using the image processing package FIJI (http://pacific.mpi-cbg.de/wiki/index.php/Fiji). Neurons are expressing soluble YFP using the neuron-specific TH1 promoter. Microglia are expressing soluble GFP (transgene inserted in the Cx3cr1 locus). The z-stack source image (provided in this image group) was acquired with an iMIC Andromeda laser spinning disc confocal microscope (http://www.till-photonics.com/Products/andromeda.php), using an Olympus UPlanSApo 60X/NA1.35 objective, and: Andor Clara (Andor) CCD Camera. Both fluorophores were excited with a 488nm laser, and so are not distinguished from each other, with these filters: Laser CleanUp, FF01-446/523/600/677 (Semrock); Dichroic, zt405/490/561/640rpc (Chroma); Emission, FF01-390/482/563/640 (Semrock). .

Biological Sources
NCBI Organism Classification
Rattus
Cell Type
multipolar neuron
microglial cell
Cellular Component
cytoplasm
axon
dendrite
apical pole of neuron
Biological Context
Biological Process
gene expression
Attribution
Names
Sebastian Rhode, TILL Photonics
Sabine Scheibe
Link
http://www.till-photonics.com/Products/androm...
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL13007
Archival Resource Key (ARK)
ark:/b7295/w9cil13007
Grouping This image is part of a group.
Imaging
Image Type
recorded image
Image Mode
spinning disk confocal microscopy
Parameters Imaged
fluorescence emission
Source of Contrast
distribution of a specific protein
Visualization Methods
EGFP
EYFP
Data Qualifiers
processed data
suitable for spatial measurements
Sample Preparation
Methods
formaldehyde fixed tissue
Relation To Intact Cell
sectioned tissue
Dimensions
Spatial Axis Image Size Pixel Size
X 1392px 0.107µm
Y 1040px 0.107µm