Licensing

Copyright
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Description

Section from rat brain cortex showing a subset of fluorescent-protein expressing neurons and microglia. Most prominent in this image is an aggregation of microglial cells surrounded by axons and dendrites coursing though the tissue section. The colors represent the Z-position in a maximum intensity projection of an image (compiled from 200 image planes, 0.1µm intervals), and were generated using the image processing package FIJI (http://pacific.mpi-cbg.de/wiki/index.php/Fiji). Neurons are expressing soluble YFP using the neuron-specific TH1 promoter. Microglia are expressing soluble GFP (transgene inserted in the Cx3cr1 locus). The z-stack source image (provided in this image group) was acquired with an iMIC Andromeda laser spinning disc confocal microscope (http://www.till-photonics.com/Products/andromeda.php), using an Olympus UPlanSApo 60X/NA1.35 objective, and: Andor Clara (Andor) CCD Camera. Both fluorophores were excited with a 488nm laser, and so are not distinguished from each other, with these filters: Laser CleanUp, FF01-446/523/600/677 (Semrock); Dichroic, zt405/490/561/640rpc (Chroma); Emission, FF01-390/482/563/640 (Semrock). .

Biological Sources

NCBI Organism Classification
Rattus
Cell Type
multipolar neuron
microglial cell
Cellular Component
cytoplasm
axon
dendrite

Biological Context

Biological Process
gene expression

Attribution

Names
Sebastian Rhode, TILL Photonics
Sabine Scheibe
Link
http://www.till-photonics.com/Products...

Grouping

This image is part of a group.

Imaging

Image Type
recorded image
Imaging Mode
spinning disk confocal microscopy
Parameters Imaged
fluorescence emission
Source of Contrast
distribution of a specific protein
Visualization Methods
EGFP
EYFP
Data Qualifiers
processed data
suitable for spatial measurements

Sample Preparation

Methods
formaldehyde fixed tissue
Relation To Intact Cell
sectioned tissue

Dimensions

Spatial Axis Image Size Pixel Size
X 576px 0.1070μm
Y 504px 0.1070μm
*CIL – Cell Image Library accession number. Please use this to reference an image.