The Cell: An Image Library

Licensing

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CIL:1265^*

Description

The purpose of the dataset C. elegans muscle aging is to deduce the age of the nemathode based on images of muscles. Images of C. elegans were taken at different chronological ages. This image is part of the day 8 data set. Note that the morphological and chronological ages are not fully correlated due to the variability among individuals even though the individuals are genetically identical. The source for the dataset is Laboratory of Genetics/NIA/NIH.

Technical Details

C. elegans muscle age is a data set of fluorescence 20X microscopy images of C. elegans nematodes stained with phalloidin to visualize actin in muscles at different ages (1,2,4, and 8). Note that the data sets for each age can be found by searching for a featured image from each set: CIL 1005, 1057, 1105, and 1265, respectively.

Biological Sources

NCBI Organism Classification: Caenorhabditis elegans; (Nematode)
Cell Type: muscle cell
Cellular Component: actin filament

Biological Context

Biological Process: chronological cell aging

Attribution

Names: Nikita Orlov; Wendy Iser; Cathy Wolkow
Link: NIH/NIA Laboratory of Genetics

Grouping

This image is representative of a group of similar images.

Imaging

Image Type: recorded image
Imaging Mode: fluorescence microscopy
Parameters Imaged: fluorescence emission
Source of Contrast: distribution of epitope
Visualization Methods: phalloidin
Processing History: unprocessed raw data

Sample Preparation

Relation To Intact Cell: whole mounted tissue

Dimensions

Spatial Axis	Image Size	Pixel Size
X	1600px	——
Y	1200px	——

*CIL – Cell Image Library accession number. Please use this to reference an image.