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Human embryonic stem cell colonies. This phase contrast image shows two human embryonic stem cell colonies growing on a feeder layer of mouse embryonic fibroblasts. The stem cells actually grow in between the fibroblasts. The feeder layer helps provide nutrition for the stem cells as well as factors that keep them from differentiating. This is a live cell preparation that was imaged using a Nikon inverted Eclipse microscope.

Biological Sources

NCBI Organism Classification
Homo sapiens
Mus musculus
(house mouse)
Cell Type
stem cell
Cellular Component

Biological Context

Biological Process
stem cell proliferation


Sabrina Lin
Prue Talbot
Stem Cell Center University of California, Riverside


This image is part of a group.


Image Type
recorded image
Imaging Mode
phase contrast microscopy
Parameters Imaged
elastic scattering of photons
Source of Contrast
differences in amount of elastic light scattering
Visualization Methods
visualization of contiguous regions
Processing History
unprocessed raw data

Sample Preparation

living tissue
Relation To Intact Cell
dispersed cells in vitro


Spatial Axis Image Size Pixel Size
X 1392px ——
Y 1040px ——
*CIL – Cell Image Library accession number. Please use this to reference an image.