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Description

Hamster oocyte and cumulus cells. This scanning electron micrograph shows a hamster oocyte. The oocyte is not directly visible but lies beneath the zona pellucida. The surface of the zona pellucida can be seen between the cumulus cells that have remained attached to it. The sample was fixed using glutaraldehyde and osmium tetroxide, dehydrated in ethanol, critically point dried, coated with gold, and examined in a Phillips XL30 FEG scanning electron microscope. Magnification � 790x.

Biological Sources

NCBI Organism Classification
Mesocricetus auratus
(Golden Hamster)
Cell Type
oocyte
cumulus cell
Cellular Component
zona pellucida

Biological Context

Biological Process
ovulation

Attribution

Name
Prue Talbot

Imaging

Image Type
recorded image
Imaging Mode
scanning electron microscopy (SEM)
Parameters Imaged
elastic scattering of electrons
Source of Contrast
differences in deposition of metal shadow
Visualization Methods
gold
Processing History
unprocessed raw data
Data Qualifiers
raw, unprocessed data

Sample Preparation

Methods
glutaraldehyde fixed tissue
osmium tetroxide fixed tissue
dehydrated in ethanol
critical_point dried specimen
Relation To Intact Cell
whole mounted tissue

Dimensions

Spatial Axis Image Size Pixel Size
X 406px ——
Y 331px ——
*CIL – Cell Image Library accession number. Please use this to reference an image.