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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:12614*  Cite 

A high resolution image of a cell actively undergoing endocytosis that develops an extensive layer of vesicles and cisternae composed for the most part of smooth undecorated membrane. The uncoated cisternae are early endosomes to which uncoated vesicles that first arise as coated vesicles from the parasomal sacs fuse with the cisternae to deposit their load of receptor-mediated cargo. Smaller clathrin-coated vesicles bud from the early endosomes and these are either recycled back to the plasma membrane or move deeper into the cell as carrier vesicles after they lose their clathrin coats. TEM taken on 5/21/79 by R. Allen with Hitachi HU11A operating at 75kV. Neg. 26,250X. The raw film was scanned with a Epson Perfection V750 Pro. This image is best used for quantitative analysis. Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. Additional information available at (

Biological Sources
NCBI Organism Classification
Paramecium multimicronucleatum
Cell Type
cell by organism
eukaryotic cell
Eukaryotic Protist
Ciliated Protist
Cellular Component
cell cortex
coated pit
clathrin vesicle coat
clathrin coat of endocytic vesicle
cell cortex
Richard Allen (University of Hawaii)
Digital Object Identifier (DOI)
Archival Resource Key (ARK)
Grouping This image is part of a group.
Image Type
transmission electron microscopy (TEM)
illumination by electrons
Image Mode
detection of electrons
Parameters Imaged
electron density
Source of Contrast
stain with broad specificity
Visualization Methods
stain with broad specificity
osmium tetroxide
uranyl salt
lead salt
Processing History
unprocessed raw data
Data Qualifiers
raw, unprocessed data
suitable for spatial measurements
Spatial Axis Image Size Pixel Size
X 4843px 0.57nm
Y 4296px 0.57nm