Licensing

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Description

Immuno-gold labeling of corn stunt spiroplasma (CSS, Spiroplasma kunkelii, Class Mollicutes) in sieve elements and other phloem cells of infected maize leaves. CIL:19126, from the same image group, is a labeled version of this image. CSS is a wall-less procaryote that causes corn stunt disease and is biologically transmitted by some leafhopper species, e.g. Dalbulus maidis and D. gelbus (Hemiptera, Cicadellidae). Spiroplasma cells are limited by a unit membrane with no cell wall. They can be quasi-spherical, pleiomorphic or maintain a filamentous/spiral form. CSS multiplies in both plant and insect hosts and is transmitted with salivary secretions during feeding of the vector on new host plants. Related images from the same contributor are also available in the Library.

Technical Details

Detailed methods: Tissue was processed for TEM by fixation in glutaraldehyde and osmium tetroxide, embedded in Spurr's medium. Thin sections were stained with uranyl acetate and lead citrate, and examined by a Philips-201 TEM (non-digital camera). Gold particles used in immunolabeling CSS were 10nm. Negatives were scanned later to generate these electronic files.

Biological Sources

NCBI Organism Classification
Spiroplasma kunkelii
Zea mays
(corn)
Cell Type
plant cell
CL:0000399
prokaryotic cell
Cellular Component
plant-type cell wall

Biological Context

Biological Process
pathogenesis

Attribution

Names
El-Desouky Ammar
Saskia A. Hogenhout

Grouping

This image is representative of a group of similar images.

Imaging

Image Type
transmission electron microscopy (TEM)
Imaging Mode
illumination by electrons
Parameters Imaged
electron density
Source of Contrast
stain with broad specificity
Visualization Methods
osmium tetroxide
lead salt
uranyl salt
gold
Data Qualifiers
processed data

Dimensions

Spatial Axis Image Size Pixel Size
X 800px 3.76nm
Y 954px 3.76nm
*CIL – Cell Image Library accession number. Please use this to reference an image.