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Copyright
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Description

NIH3T3 cells showing the localization of nuclear transport factor RanBP1 and F-actin in interphase cells. Cells were fixed in formaldehyde and labeled with a primary antibody to RanBP1 and Cy3-conjugated secondary to (RanBP1), FITC-labelled phalloidin, and DAPI. Images were collected on a Zeiss LSM510 confocal using a scan speed 6, 8-line averaging and a 63X 1.4NA objective.

Biological Sources

NCBI Organism Classification
Mus musculus
(house mouse)
Cell Type
fibroblast
Cell Line
NIH/3T3
Cellular Component
actin cytoskeleton
nucleus
nuclear transport factor RanBP1

Attribution

Names
Hicham Mahboubi
Ursula Stochaj
Data Qualifiers
raw, unprocessed data

Sample Preparation

Methods
formaldehyde fixed tissue
Relation To Intact Cell
dispersed cells in vitro

Dimensions

Spatial Axis Image Size Pixel Size
X 1024px 0.8μm
Y 1024px 0.8μm
Channel Wavelength
1 DAPI, FITC, CY3 nm
*CIL – Cell Image Library accession number. Please use this to reference an image.