Cell of the DT40 chicken B lymphocyte line at mitosis. Z-series of widefield immunofluorescence images captured using a 100x/1.4NA planapo objective lens on a Deltavision system and deconvolved with a constrained iterative algorithm (Applied Precision). Displayed is the central z-slice. Prometaphase localization of INCENP (red) in INCENP-OFF cells expressing TrAP-INCENP F802A. Endogenous Survivin is green and DAPI (DNA) is blue. See Supplementary Fig 2D panels 1-4 in Supplementary data for Xu et al. 2009 J Cell Biol 187, 637-653.