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CIL:12027*

Description

Movie of meiosis I, starting at metaphase in primary spermatocytes of the crane-fly Nephrotoma suturalis that have been experimentally flattened. Time-lapse polarization microscopy using a Nikon Microphot SA, equipped for liquid crystal polarized light microscopy (LC-PolScope, CRi, Woburn Massachusetts) 60x/1.4 PlanApo oil immersion objective, 1.4 NA oil immersion condenser, with 2.0x zoom lens. Images captured every 15 sec over 35 min using a QImaging Retigo EXi CCD camera. Raw images were processed using 5-frame algorithm (Shribak and Oldenbourg, 2003). The time series used for the movie is included in this grouped set.

Biological Sources

NCBI Organism Classification
Nephrotoma
(Tiger Crane Flies)
Nephrotoma suturalis
Cell Type
primary spermatocyte
Cellular Component
spindle
nuclear chromosome

Biological Context

Biological Process
male meiosis chromosome segregation

Attribution

Names
James R. LaFountain
Rudolf Oldenbourg
Publisheds
LaFountain JR, et al. (2004) Mol Cell Biol 15, 5724-5732
Oldenbourg R, Mei G (1995) J Microsc 180, 140-147.
Shribak M, Oldenbourg R (2003) Appl Opt 42, 3009-3017
Pubmed
15469981
8537959
12790452

Grouping

This image is part of a group.

Imaging

Image Type
charge coupled device (CCD)
Imaging Mode
polarization microscopy
Parameters Imaged
UV/visible/IR photons
Source of Contrast
intrinsic birefringence
Visualization Methods
relative retardation (retardance) measurement
Processing History
5-frame algorithm

Sample Preparation

Methods
living tissue
Relation To Intact Cell
whole mounted tissue

Dimensions

Spatial Axis Image Size Pixel Size
X 678px 87nm
Y 518px 87nm
Time 15 sec 140
*CIL – Cell Image Library accession number. Please use this to reference an image.