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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:11958*  Cite 
Description

This is one of 4 grouped time lapse movies (CIL 11958-11962) revealing the responses of a meiotic oocyte spindle to various forms of micromanipulation by a glass microneedle. The needle is inserted, from below, through the egg cortex to the inner pole of the metaphase spindle. This movie, speeded up about 10-fold, shows the transient puckering in the cell membrane showing that the spindle pole is attached to the cortex. After the attachment has been broken, the cell surface rounds back up. The spindle is made visible by the use of polarizing optics.

Technical Details

See DA Lutz et al 1988. Micromanipulation of the asymmetric positioning of the maturation spindle in Chaetopterus sp oocytes. Cell Motil Cytoskel 11:83-96. This paper includes stills of similar experiments.

Biological Sources
NCBI Organism Classification
Chaetopterus sp. 1 Woods Hole MA
Cell Type
oocyte
Cellular Component
spindle
Biological Context
Biological Process
meiosis
Attribution
Names
Shinya Inoue
Published
S. Inoue 2008 Collected Works of Shinya Inoue. World Scientific Publishing Company, Singapore, Hackensack, NJ, USA
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL11958
Archival Resource Key (ARK)
ark:/b7295/w9cil11958
Grouping This image is part of a group.
Imaging
Image Type
recorded image
Image Mode
polarization microscopy
Parameters Imaged
retardance
Source of Contrast
form birefringence
Visualization Methods
visualization of contiguous regions
Data Qualifiers
processed data
Sample Preparation
Methods
living tissue
Relation To Intact Cell
whole mounted tissue
Dimensions
Spatial Axis Image Size Pixel Size
X 704px ——
Y 480px ——