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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:11946*  Cite 
Description

The movie shows two sequences that begin with an unfertilized Arbacia (sea urchin) egg in which the contents have been stratified using the centrifuge polarizing microscope (CPM) developed by S. Inoue. The main feature seen with the polarizing microscope is the appearance of a negatively birefringent layer of stacked membranes. Sperm are then introduced. A few seconds after sperm entry, this birefringence disappears as stacked membranes fragment. The egg also becomes lighter and rises.

Technical Details

See:S Inoue et al. 2001. Centrifuge polarizing microscope II. Sample biological applications. J Microsc 201:357-367.

Biological Sources
NCBI Organism Classification
Arbacia punctulata
Cell Type
oocyte
Biological Context
Biological Process
fertilization
Attribution
Names
Shinya Inoue
Published
S. Inoue 2008 Collected Works of Shinya Inoue. World Scientific Publishing Company, Singapore, Hackensack, NJ, USA
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL11946
Archival Resource Key (ARK)
ark:/b7295/w9cil11946
Imaging
Image Type
recorded image
Image Mode
polarization microscopy
Parameters Imaged
retardance
Source of Contrast
form birefringence
Visualization Methods
visualization of contiguous regions
Data Qualifiers
processed data
Sample Preparation
Methods
living tissue
Relation To Intact Cell
whole mounted tissue
Dimensions
Spatial Axis Image Size Pixel Size
X 720px ——
X 480px ——


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