Licensing

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Description

The time-lapse movie shows early development in the Lytechinus (sea urchin) embryo. Polarizing microscopy highlights the mitotic spindles which may show bright or dark contrast according to their orientation with respect to the polarized light. For clearer visualization, the embryo was flattened - this leads to abnormal spatial location of cells at the later divisions.

Technical Details

See: Inoue S (1964) Organization and function of the mitotic spindle. In, Primitive Motile systems in Cell Biology. pp549-598. Academic Press

Biological Sources

NCBI Organism Classification
Lytechinus
Cell Type
early embryonic cell

Biological Context

Biological Process
mitosis

Attribution

Names
Shinya Inoue
F. Rogier
Published
S. Inoue 2008 Collected Works of Shinya Inoue. World Scientific Publishing Company, Singapore, Hackensack, NJ, USA

Imaging

Image Type
recorded image
Imaging Mode
polarization microscopy
Parameters Imaged
retardance
Source of Contrast
form birefringence
Visualization Methods
visualization of contiguous regions
Data Qualifiers
processed data

Sample Preparation

Methods
living tissue
Relation To Intact Cell
whole mounted tissue

Dimensions

Spatial Axis Image Size Pixel Size
X 720px ——
Time -- sec
*CIL – Cell Image Library accession number. Please use this to reference an image.