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Figure 302 from Chapter 12 (Centrioles) by Don Fawcett. The centrioles replicate early in cell division and take up positions at either pole of the division figure. Concurrently with the condensation of the chromosomes and breakdown of the nuclear envelope, microtubules polymerize, extending from the chromosomes to the poles and from pole to pole, to form the mitotic spindle. In the accompanying micrograph made before introduction of aldehyde fixatives, the microtubules have not been well preserved. The plane of section is a fortunate one, however, in that three of the centrioles are included. The second centriole at the lower pole is in a plane perpendicular to this section and is not seen. Because the two diplosomes usually differ in their orientation, all four centrioles are rarely, if ever, included in the same thin section. A copy of the chapter is available on the ASCB's BioEDUCATE website.

Biological Sources

NCBI Organism Classification
Gallus gallus
(red junglefowl)
Cell Type
Cellular Component
pericentriolar material
nuclear chromosome

Biological Context

Biological Process
spermatogonial cell division


Don W. Fawcett
Toshio Nagano
ISBN 0721635849


This image is part of a group.


Image Type
transmission electron microscopy (TEM)
illumination by electrons
Imaging Mode
detection of electrons
Parameters Imaged
electron density
Source of Contrast
stain with broad specificity
Visualization Methods
stain with broad specificity
osmium tetroxide
uranyl salt
lead salt
Processing History
recorded image
Print from negative scanned for Photoshop.
Data Qualifiers
processed data


Spatial Axis Image Size Pixel Size
X 920px ——
Y 1292px ——
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