Alternate header for print version


Licensing
Attribution Non-Commercial; No Derivatives:This image is licensed under a Creative Commons Attribution, Non-Commercial, No Derivatives License. View License Deed | View Legal Code
tweet  
Comments: Powered by Pnyxe
*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:10900*  Cite 
Description

This electron micrograph shows the E-fracture face (above) and P-fracture face (below) of the membrane of adjacent epithelial cells from the ciliary epithelium of the monkey eye. Intramembranous particles, representing integral membrane proteins, appear as bumpy projections, and are prominent in this image with high density on the p-face, but also can be detected on the e-face. This image, contributed by Giuseppina Raviola, is Figure 4 from Chapter 1 (The Cell Surface) of 'The Cell, 2nd Ed.' by Don W. Fawcett M.D. A PDF copy of the accompanying chapter is available on the ASCB's BioEDUCATE website.

Technical Details

With the freeze-fracture technique, tissue is rapidly frozen and cracked to shear along zones of weakness. Cleavage of membranes occurs along the hydrophobic interior of the lipid bilayer to reveal views of a "p-face" (the outwardly-facing inner half of the membrane) and an "e-face" (the inwardly-facing outer half of the membrane), and a metallic replica is made of the fractured surface.

Biological Sources
NCBI Organism Classification
unidentified monkey
Cell Type
epithelial cell
Cellular Component
plasma membrane
intrinsic to plasma membrane
Biological Context
Biological Process
plasma membrane organization
Attribution
Names
Giuseppina Raviola
Published
ISBN 0721635849
Link
The Cell, 2nd Ed. by D.W. Fawcett
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL10900
Archival Resource Key (ARK)
ark:/b7295/w9cil10900
Grouping This image is part of a group.
Imaging
Image Type
transmission electron microscopy (TEM)
Image Mode
illumination by electrons
detection of electrons
Parameters Imaged
electron density
Source of Contrast
differences in deposition of metal shadow
Visualization Methods
shadowing and plating
Data Qualifiers
processed data
Sample Preparation
Methods
freeze_fracture/freeze_etch
Relation To Intact Cell
isolated subcellular component
Dimensions
Spatial Axis Image Size Pixel Size
X 898px ——
Y 1295px ——


Ascb_logo
Ccdb_logo_banner
Nif_button2
Dci_button
Labbyrcw