Licensing

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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:10705^* Cite

Description

High voltage TEM image of meiotic newt oocyte nuclei showing transcription on looped lampbrush chromosomes. This micrograph, was recorded at a tilt angle of 50 degrees. The image is grouped with one of the same sample area recorded at a 40 degree tilt. A stereo examination of the pair provides an oblique 3D view.

Technical Details

Oocytes were allowed to disperse in 30 mM KCl/NaCl, centrifuged through 2% formaldehyde onto an EM grid, stained with uranyl acetate, and critical point dried. Grids were examined with the Madison 1MeV TEM at 5 KX.

Biological Sources

NCBI Organism Classification: Notophthalmus viridescens
Cell Type: oocyte
Cellular Component: lampbrush chromosomes

Biological Context

Biological Process: Transcription; chromosome organization; RNA metabolic process

Attribution

Names: Hans Ris

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL10705
Archival Resource Key (ARK): ark:/b7295/w9cil10705

Grouping This image is part of a group.

Imaging

Image Type: film
Image Mode
Parameters Imaged: electron density
Source of Contrast: differences in adsorption or binding of stain
Visualization Methods: uranyl salt
Processing History: unprocessed raw data

Sample Preparation

Methods: formaldehyde fixed tissue
Relation To Intact Cell: isolated subcellular component

Dimensions

Spatial Axis	Image Size	Pixel Size
X	5984px	2.5nm
Y	4152px	2.5nm