The Cell: An Image Library - Image CIL:10566

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CIL:10566^*

Description

A monolayer of human foreskin fibroblast cells was infected with Toxoplasma gondii expressing YFP-tagged Tgalpha1tubulin and mRFP-tagged TgDLC (dynein light chain). DIC Images acquired using API DeltaVision on a Olympus IX70 inverted microscope. Objective: Olympus 60X, 1.4 NA. Captured with CoolSnapHQ/ICX285. DIC stack corresponds to raw data stack: CIL# 10562. Unpublished image that is similar to images published in PMID: 16518471. See publication for other experimental details.

Biological Sources

NCBI Organism Classification: Toxoplasma gondii RH
Cell Line: Toxoplasma gondii RH stable cell line
Cellular Component: dynein complex; centriole; spindle pole; conoid; cortical microtubule

Biological Context

Biological Process: regulation of cell shape; microtubule-based movement; microtubule cytoskeleton organization
Molecular Function: ATPase activity, coupled; motor activity; structural constituent of cytoskeleton
Human Disease: toxoplasmosis

Attribution

Names: John Murray (University of Pennsylvania); Ke Hu (Indiana University, Bloomington)

Grouping

This image is part of a group.

Imaging

Image Type: recorded image; charge coupled device (CCD)
Imaging Mode: differential interference contrast microscopy
Parameters Imaged: optical path length gradient
Source of Contrast: boundaries between regions with different refractive index

Data Qualifiers: raw, unprocessed data; suitable for spatial measurements

Sample Preparation

Methods: living tissue
Relation To Intact Cell: whole mounted tissue

Dimensions

Spatial Axis	Image Size	Pixel Size
X	384px	.0710μm
Y	384px	.0710μm
Z	35	.2μm

*CIL – Cell Image Library accession number. Please use this to reference an image.