Licensing

Pd
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Description

A monolayer of human foreskin fibroblast cells was infected with Toxoplasma gondii expressing YFP-tagged TgIMC1 (innermembrane complex scaffold protein) and mCherry-tagged human alpha1tubulin. Images acquired using API DeltaVision on a Olympus IX70 inverted microscope. Objective: Olympus 60X water immersion, 1.2 NA. Filters: mCherry, ex 555-592 em 600-665, YFP, ex 490-510 em 525-595. Captured with CoolSnapHQ/ICX285. This data stack corresponds to data stack: CIL# 10538, deconvolved data stack: CIL# 10539 and maximum intensity projections: CIL# 10540 and CIL# 10541. Unpublished image that is similar to images published in PMID: 16518471. See publication for other experimental details.

Biological Sources

NCBI Organism Classification
Toxoplasma gondii RH
Cell Line
Toxoplasma gondii RH stable cell line
Cellular Component
cortical microtubule
spindle pole
centriole
conoid
inner membrane complex

Attribution

Names
John Murray (University of Pennsylvania)
Ke Hu (Indiana University, Bloomington)

Grouping

This image is part of a group.
Data Qualifiers
raw, unprocessed data
suitable for spatial measurements

Sample Preparation

Methods
living tissue
Relation To Intact Cell
whole mounted tissue

Dimensions

Spatial Axis Image Size Pixel Size
X 1024px .1067μm
Y 1024px .1067μm
Z 17 .3μm
*CIL – Cell Image Library accession number. Please use this to reference an image.