A monolayer of human foreskin fibroblast cells was infected with Toxoplasma gondii expressing EGFP-tagged Tgbeta3-tubulin. 50 timepoints in which daughter formation is visible. Images overlaid with DIC. Images acquired using API DeltaVision on an Olympus IX70 inverted microscope. Objective: Olympus 60X water immersion, 1.2 NA. Filters: GFP, ex 450-490 em 500-540. Captured with CoolSnapHQ/ICX285. 3D stack is raw data corresponding to deconvolved stack: CIL# 10489. Unpublished image that is similar to images published in PMID: 16518471. See publication for other experimental details.
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