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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:10276*  Cite 
Description

After over-night serum starvation, mouse fibroblast expressing Dyn2-GFP were stimulated with 10ng/ml PDGF. After 5min, the cells were fixed and stained with rhodamine-phalloidin. PDGF-induced dorsal membrane ruffle was indicated by F-actin staining, and the enrichment of dynamin to the membrane ruffle was shown by their co-localization. Biological Source Mus musculus ES cell derived fibroblast PDGF-induced macropinocytosis PFA fixation Biological Context and Probe PDGF-induced dorsal membrane ruffle, Dyn2-GFP and rhodamine-phalloidin Equipment used Inverted Olympus IX-70 microscope with a 100×, 1.35 NA oil-immersion objective Magnification of image 1000x, scale bar indicates 5 um

Biological Sources
NCBI Organism Classification
Mus musculus
Cell Type
fibroblast
Cellular Component
dynamin
actin cytoskeleton
dorsal membrane ruffle
Attribution
Names
Ya-Wen Li
Sandra L. Schmid
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL10276
Archival Resource Key (ARK)
ark:/b7295/w9cil10276
Imaging
Image Type
recorded image
Image Mode
fluorescence microscopy
Parameters Imaged
fluorescence emission
Source of Contrast
distribution of a specific protein
Visualization Methods
phalloidin
Rhodamine
EGFP
Processing History
unprocessed raw data
Sample Preparation
Relation To Intact Cell
dispersed cells in vitro
Dimensions
Spatial Axis Image Size Pixel Size
X 801px 0.059µm
Y 770px 0.059µm
Channel Wavelength
1 GFP, Rhodamineµm


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