Licensing

Copyright
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Description

After over-night serum starvation, mouse fibroblast expressing Dyn2-GFP were stimulated with 10ng/ml PDGF. After 5min, the cells were fixed and stained with rhodamine-phalloidin. PDGF-induced dorsal membrane ruffle was indicated by F-actin staining, and the enrichment of dynamin to the membrane ruffle was shown by their co-localization. Biological Source Mus musculus ES cell derived fibroblast PDGF-induced macropinocytosis PFA fixation Biological Context and Probe PDGF-induced dorsal membrane ruffle, Dyn2-GFP and rhodamine-phalloidin Equipment used Inverted Olympus IX-70 microscope with a 100×, 1.35 NA oil-immersion objective Magnification of image 1000x, scale bar indicates 5 um

Biological Sources

NCBI Organism Classification
Mus musculus
(house mouse)
Cell Type
fibroblast
Cellular Component
dynamin
actin cytoskeleton
dorsal membrane ruffle

Attribution

Names
Ya-Wen Li
Sandra L. Schmid

Imaging

Image Type
recorded image
Imaging Mode
fluorescence microscopy
Parameters Imaged
fluorescence emission
Source of Contrast
distribution of a specific protein
Visualization Methods
phalloidin
Rhodamine
EGFP
Processing History
unprocessed raw data

Sample Preparation

Relation To Intact Cell
dispersed cells in vitro

Dimensions

Spatial Axis Image Size Pixel Size
X 801px 0.059μm
Y 770px 0.059μm
Channel Wavelength
1 GFP, Rhodamine μm
*CIL – Cell Image Library accession number. Please use this to reference an image.