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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:10006*  Cite 
Description

Daughter micronucleus in anaphase of a dividing cell of Didinium nasutum. The chromosomes are compacted into one large heterochromatic body. Remnants of the mitotic division spindle remain inside the intact nuclear envelope. Nuclear envelopes remain intact in ciliates during division in both the micronucleus and macronucleus. Mitochondrion, peroxisome, dictyosomes of Golgi complex, and rough endoplasmic reticulum are evident. TEM taken on 5/19/69 by R. Allen with Philips 300. Neg. 14,800X. Bar = 0.5µm. The negative was printed to paper and the image was scanned to Photoshop. This digitized image is available for qualitative analysis. A raw, unprocessed, high resolution version of this image (CIL:4662) is in the library and available for quantitative analysis. Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).

Biological Sources
NCBI Organism Classification
Didinium nasutum
Cell Type
cell by organism
eukaryotic cell
Eukaryotic Protist
Ciliated Protist
Cell Line
Carolina Biological Supply Company, NC, U.S.A.
Cellular Component
micronucleus
nuclear heterochromatin
condensed nuclear chromosome
nuclear envelope
spindle microtubule
Biological Context
Biological Process
mitotic anaphase
Attribution
Names
Richard Allen (University of Hawaii)
Link
http://www5.pbrc.hawaii.edu/allen/
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL10006
Archival Resource Key (ARK)
ark:/b7295/w9cil10006
Grouping This image is part of a group.
Imaging
Image Type
transmission electron microscopy (TEM)
illumination by electrons
Image Mode
detection of electrons
film
Parameters Imaged
electron density
Source of Contrast
stain with broad specificity
Visualization Methods
stain with broad specificity
osmium tetroxide
uranyl salt
lead salt
Processing History
recorded image
film
Print from negative scanned for Photoshop.
Data Qualifiers
processed data
suitable for spatial measurements
Dimensions
Spatial Axis Image Size Pixel Size
X 3469px ——
Y 2872px ——