Contractile vacuole in the opisthe of a dividing cell. This is presumably the mature CV of the original “mother” cell. Spongiome tubules form large regions of additional membrane on two sides of t...

Conventional freeze-fractured images of DV-III membrane also suggests a transformation from a membrane with a smooth E-fracture face to one with characteristic large IMPs on its E-fracture face. The D...

Quick-freeze deep-etch image of a DV-III containing paracrystalline sheets in its lumen. The sheets have parallel rows of subunits spaced at 20nm center-to-center but the chemical nature of the sheets...

A partially filled contractile vacuole is connected to two regions of spongiome tubules. The extra CV membrane is acquired from these spongiomes. Presumably some of these spongiome tubules are decorat...

In more extreme cases microfilamentous material builds up into a thick layer surrounding the DV-I membrane as well as the fusion sites between the acidosomes and the DV-I. A similar thick meshwork was...

Secondary lysosomes dock only with phagoacidosome membrane at a distance of 40nm. As during acidosome fusion with phagosomes, fusion of the many lysosomes with the phagoacidosome occurs at nearly the ...

A mAb, B25-1-3, was raised to antigen Q2 (arbitrary code) to the same set of membranes as mAb 132-F4-G6 labels. This mAb was more reactive on frozen sections. To tell the age of vacuoles, cells were e...

Two of the three types of vesicles that are transported to the cytopharynx and that accumulate in large numbers at the cytopharynx are shown in this electron micrograph. The discoidal vesicles move up...

A freeze-fracture image of a DV-II transforming into a DV-III by rapid fusion of lysosomes with its membrane. The P-face shown here of the early DV-III has abundant IMPs as well as many inward blebs (...

As with acidosome fusion with the DV-I, lysosome fusion with the DV-II also seems to occur nearly all at once resulting in a phagolysosome (DV-III) with a highly irregular surface. However, unlike the...