To see if acidosomes contain hydrolases (digestive enzymes) we incubated cells in Gomori’s medium which in the presence of acid phosphatase and lead will form an electron-opaque reaction product. Ac...

The acidosome-like vesicle has at least 2 enclosing membranes or one membrane indented back on itself and an internal electron-opaque tongue-like droplet that is continuous with the enclosing membrane...

The cytosol of Paramecium contains many vesicles of various sizes, shapes and contents as well as complex particles such as ribosomes and glycogen granules. The rough endoplasmic reticulum exists as m...

The bottom of the buccal cavity is surrounded by specialized cytoplasm and contains a lip that constricts the opening leading through the cytostome into the receiving vacuole or nascent vacuole. The ...

This is one of a group of four images in Figure S5 in Apaja et al., JCB 2010, that support the conclusion that the WT vasopressin type2 receptor (V2R) and the V2R W164S mutant (that causes nephrogenic...

Secondary lysosomes contain a lumen in quick-freeze deep-etch (QF-DE) images resembling etched matrix proteins rather than the honeycomb appearance of acidosomes. Lysosomes often have one or more para...

The acidosome-like vesicle has a bull’s eye appearance with the invagination appearing in the middle of the vesicle. Other vesicles contain multiple layers resembling multilipid layers. Two vesicles...

In more extreme cases microfilamentous material builds up into a thick layer surrounding the DV-I membrane as well as the fusion sites between the acidosomes and the DV-I. A similar thick meshwork was...

Secondary lysosomes dock only with phagoacidosome membrane at a distance of 40nm. As during acidosome fusion with phagosomes, fusion of the many lysosomes with the phagoacidosome occurs at nearly the ...

Vorticella convallaria fixed for EM and buffered in cacodylate. The rough ER is not vesiculated as it is in collidine buffered fixatives but appears as sheets from which Golgi dictyosomes arise. A foo...