Spinning disk confocal time-lapse imaging of the first mitosis in a C. elegans embryo that has been depleted of KNL-2 by RNAi. GFP-histone and GFP-γ-tubulin were imaged (strain TH32). Pronuclear migr...

Spinning disk confocal time-lapse imaging of the first mitosis in a C. elegans embryo expressing GFP-KNL-2 in strain OD31. As seen for GFP-CeCENP-A (CIL 28780), discrete foci of the GFP signal within ...

Nuclear envelope and spindle dynamics when the nucleus moves into the neck of kar9delta mutant cells arrested in S phase. Movie plays at 100x real time and is movie S6 in Proc Natl Acad Sci. 2009. 10...

Time lapse movie of a porcine kidney LLCPK1 cell stably expressing photoactivable GFP-tubulin. Fluorescence was activated at prophase in a vertical strip perpendicular to the spindle orientation. Mic...

The JNK phosphatase puckered, whose expression can be reported via a lacZ enhancer trap, is expressed in very low levels in uninjured neurons; however, nerve crush induces a dramatic increase in puc-l...

A confocal 4D-stack from a transgenic Drosophila embryo expressing lamin-GFP (green) and injected with rhodamine-conjugated tubulin (red) and anti-lamin-B antibodies during prometaphase. The microinje...

Nuclear envelope and spindle dynamics when the nucleus moves into the neck of kar9delta Nip100deltaCAP-Gly double mutant cells arrested in S phase. In these cells, the spindle failed to enter the bud ...

Spindle oscillation in wild-type cells arrested in S phase. In wild-type cells, the spindle moves in both directions through the bud neck; these movements coincide with lateral sliding of a cytoplasmi...

Spindle oscillation in kar9delta cells arrested in S phase. In wild-type cells, the spindle moves in both directions through the bud neck; these movements coincide with lateral sliding of a cytoplasmi...

Timelapse movie of GFP-labeled microtubules in kar9delta cells (arrested in S-phase) combined with a deletion of the CAP-Gly domain of Nip100. The number of cells in which the spindle moved from the m...