The nascent digestive vacuole forms as the membrane of flattened vesicles fuse with the single membrane of the cytopharynx between the lamellae (2 microtubules forming a lamella) that are connected al...

Electron micrograph of PC12 cells transfected with control siRNA. PC12 cells were plated onto aclar film discs (Pella) coated with poly-L-lysine, transfected twice with 50 nM control siRNA and fixed w...

Electron micrograph of PC12 cells transfected with control siRNA. PC12 cells were plated onto aclar film discs (Pella) coated with poly-L-lysine, transfected twice with 50 nM control siRNA and fixed w...

Electron micrograph of PC12 cells transfected with control siRNA. PC12 cells were plated onto aclar film discs (Pella) coated with poly-L-lysine, transfected twice with 50 nM control siRNA and fixed w...

Electron micrograph of PC12 cells transfected with control siRNA. PC12 cells were plated onto aclar film discs (Pella) coated with poly-L-lysine, transfected twice with 50 nM control siRNA and fixed w...

Electron micrograph of PC12 cells transfected with AP-3 siRNA. PC12 cells were plated onto aclar film discs (Pella) coated with poly-L-lysine, transfected twice with 50 nM AP-3delta1 siRNA and fixed w...

In some cells exposed to horseradish peroxidase (HRP) for 30 sec and then immediately fixed some HRP has already entered the cell by endocytosis and has been removed from the early endosomes in carrie...

Quick-freeze deep-etch images show a more three dimensional view of acidosomes surrounding a phagosome. Acidosomes are unique in that they contain indentations of their membrane. The lumen has a honey...

A high magnification view of the intimate docking of acidosomes with the phagosome is particularly apparent where the P-face of the acidosome lies against the E-face of the phagosome. Numerous large I...

The cytopharynx/nascent digestive vacuole (phagosome) has an E-fracture face containing a high number of IMPs. Interestingly these IMPs are sometimes organized into plaques where there are several sho...