Association of plectin with myosin II. A gelsolin-treated REF-52 cytoskeleton immunogold labeled myosin II (10 nm). After actin depletion, intermediate filaments with plectin sidearms remain associate...

Time-lapse microscopy of a spreading MIIB (myosin II B)-deficient cell cell expressing RLC-D,D–GFP (a phospho-mimetic mutant of the RLC) on fibronectin. Arrows point to clusters, or proto-bundles, d...

MT inhibition alters the location of the cell front. Time-lapse of a cell expressing RLC-D,D–GFP (a phospho-mimetic mutant of the RLC) on fibronectin in the presence of 1 mM nocodazole. Note the acc...

6 MIIB inhibits adhesion turnover. Time-lapse of a CHO.K1 cell cotransfected with very low levels of GFP-MIIB (myosin IIB, green) and paxillin (magenta). Note that adhesions adjacent to MIIB-containin...

Association of plectin with myosin II. A gelsolin-treated REF-52 cell immunogold labeled for plectin (10 nm). After actin depletion, intermediate filaments with plectin sidearms bind to clusters of my...

Time-lapse of a CHO.K1 cell cotransfected with very low levels of GFP-MIIA (myosin IIA, green) and paxillin (magenta). Yellow arrowheads mark paxillin-containing adhesions that disassemble concomitant...

Association of plectin with myosin II. A gelsolin-treated REF-52 cell immunogold labeled for plectin (10 nm). After actin depletion, intermediate filaments with plectin sidearms remain associated with...

To study the molecular mechanism by which nonmuscle myosin II (MII) regulates protrusion and adhesion dynamics in migrating cells, NIH3T3 cells were treated with scrambled siRNA (Scr) for 2 days, trea...

To study the molecular mechanism by which nonmuscle myosin II (MII) regulates protrusion and adhesion dynamics in migrating cells, NIH3T3 cells were treated with βPIX siRNA for 2 days, treated with b...

Association of plectin with myosin II. Platinum replica of nocodazole and treated REF-52 cell with actin depleted by gelsolin and plectin labeled with immunogold (10 nm). Individual plectin molecules...