Phase contrast image of PtK1 cell (marsupial kidney epithelial cell) injected with Pak1 inhibitory fragment (H83L), X-rhodamine tubulin, and expressing constitutively active Rac(Q61L). Microtubule dyn...

Phase contrast of a PtK1 cell (marsupial kidney epithelial cell) injected with Pak1 inhibitory fragment (H83L, 120 uM needle concentration) and X-rhodamine tubulin. Microtubules dynamics in these cell...

Microtubules in a Rac1(Q61L)-expressing PtK1 cell injected with Pak inhibitory fragment PBD/ID(H83L). Microtubules do not exhibit retrograde flow and undergo very little net growth. Epifluorescence i...

Actin dynamics in a Rac1(Q61L)-expressing PtK1 cell injected with Pak inhibitory fragment PBD/ID(H83L). Fast retrograde flow still occurs in the lamellipodium, but the width of the lamellipodium is re...

Phase contrast image of a PTK-1 cell expressing constitutively active Rac1(Q61L). Live cells were visualized using X-rhodamine tubulin (injected at 1 mg/mL)and eGFP-Rac1(Q61L) (injected into the nucl...

Phase contrast image of control PtK1 cell whose microtubules were visualized by microinjected X-rhodamine tubulin and fluorescence microscopy. This cell corresponds to Figure 1 and Video 1 of J Cel...

Microtubules in a control PtK1 cell at the edge of an epithelial cell island. Few pioneer microtubules rapidly grow into forming protrusions. The cell was microinjected with X-rhodamine-conjugated tub...

Microtubule fragments exhibiting net treadmilling in a Rac1(Q61L)-expressing PtK1 cell. Microtubule plus ends undergo dynamic instability while their minus ends depolymerize, resulting in net treadmil...

Differential interference microscopy (DIC) image of PtK1 cell (marsupial kidney epithelial cell expressing dominant-negative Rac1(T17N). Live cells were used to study microtubule dynamics using X-rhod...

Microtubules in a PtK1 cell injected with the Pak inhibitory fragment PBD/ID(H83L). Microtubules do not exhibit retrograde flow and undergo very little net growth. Epifluorescence image series were ac...