Stacked raw data in IMOD format. As stated in the publication, the data was taken at a variety of magnifications. The earliest version obtainable was already cropped and prealigned.
Study of morphology of quantitative morphology of mitochondria in the peripheral nerve of the adult rat using 3D electron microscopy
Funding agency
National Institute of Neurological Disorders and Stroke, National Center for Research Resources, National Institute of Diabetes and Kidney Diseases, National Library of Medicine
Leader(s)
Mark H. Ellisman
Guy A. Perkins
Start date
unspecified
End date
05-18-2010
Experiment
Experiment ID
8114
Title
Experiment 1
Purpose
Comparison of high pressure freezing and conventional fixation on mitochondrial structure using electron tomography
Experimenter(s)
Guy Perkins
Microscopy product
Microscopy product ID
8154
Instrument
JEOL4000EX
Microscopy type
IVEM
Product type
SINGLE TILT
Image basename
hpfyingsprnew
Subject
Species
Rat
Scientific name
Rattus norvegicus
Strain
Sprague Dawley
Age
1 months
Age class
young adult
Tissue section
Anatomical location
spinal root
Microtome
Ultramicrotome
Specimen description
System
peripheral nervous system
Structure
mitochondrion
Imaging parameters
Type
Electron microscopy product
Magification
0
Specimen preparation
Protocol used
Chemical fixation with 2% paraformaldehyde and 2.5% glutaraldehyde via vascular perfusion in 0.15M cacodylate buffer (pH 7.4) at 37 degrees C followed by high pressure freezing and freeze substitution using a Bal Tec HPM010 high pressure freezer (from Perkins et al., J. Structural Biology 161, 469, 2008).
Imaging product type
Type
Single tilt
Description
singlet_desc
Min range
-60 degrees
Max range
60 degrees
Tilt increment
2 degrees
Citation Information
Mark H. Ellisman, Guy A. Perkins CCDB:8154, Rattus norvegicus, mitochondrion. CIL. Dataset. https://doi.org/doi:10.7295/W9CCDB8154
