Large scale mosaic of mouse cerebellum reconstructed through automated acquisition and stitching of over 1700 individual tiles, triple labeled for GFAP (Red), Connexin 43 (Green) and DAPI (Blue), and imaged using multiphoton microscopy. The contrast and brightness was adjusted on the thumbnail image for display purposes and is different on the downloadable and Zoomify data.
Full resolution image description
1,776 tiled images were stitched together to reconstruct this single, high-resolution mosaic image of the cerebellum within a sagittal section of the left hemisphere of the mouse brain. Red is Rhodamine RedX labeling of Chicken anti-GFAP. Green is Alexafluor488 labeling of Rabbit anti-Connexin43. Blue is DAPI staining of all nuclei.
A representative tile from a large mosaic: this thumbnail image is a projection of 4 Z-slices from 3 fluorescent channels acquired from brain tissue using a 2-photon confocal microscope. Red is Rhodamine RedX labeling of Chicken anti-GFAP. Green is Alexafluor488 labeling of Connexin43. Blue is DAPI staining of all nuclei.
Full resolution image description
This raw data file contains 7,104 3-color images (37x48x4) that make up the 1,776 tiles of this brain montage. A 50 micron thick sagittal section of the left hemisphere of the mouse brain was labeled with an antibody against Connexin43 protein and an antibody against GFAP protein. The nuclei of the brain tissue were labelled with DAPI staining. The area of the entire cerebellum was imaged using a 2-photon confocal microscope.
A neuroinformatics approach to pannexin expression, localization, and interaction with other brain proteins. This research aims to examine brain tissue at high resolution and, in combination with developing neuronformatics tools, to probe for binding partners and signalling partners to investigate the pannexin "interactome". Data submissions for this project will consist of wide-scale high resolution confocal mosaic images of triple-labeled whole mouse brain sections. Pannexin 1 expression will be compared to that of pannexin 2 as well as the connexins. Other putative interaction partners of interest include the P2 purinergic receptors and components of the cytoskeleton.
