Tomographic reconstruction of a spiny dendrite from mouse hippocampus stained by double impregnation with copper and lead salts and imaged using most probable loss energy filtering (see Bouwer et al., 2004). This staining technique also fills membrane bound organelles such as synaptic vesicles with electron dense reaction product.
Full resolution image description
files were aligned in IMOD and reconstructed using TxBr with quadratic corrections