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Cell Process
Cell Component
Cell Type
Organism
Microbial
Alzheimer's
Data Sets
University of California, San Diego
9500 Gilman Drive
La Jolla, CA 92093-0608, USA
Voice
: (858) 534-0276
Fax
: (858) 534-7497
Email
: dorloff@ncmir.ucsd.edu
Reconstruction
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Single slice through a double tilt tomogram of the paranodal region of the Node of Ranvier from mouse sciatic nerve
Full resolution image description
Tomogram of the paranodal region of a peripheral nerve node of Ranvier
Volume_dimension
944, 1024, 200
Volume scale
0.005, 0.005, 0.0075
Animation description
Tomographic reconstruction with annotations of the paranodal glial-axonal junction from mouse peripheral nerve, using double tilt tomography
Image 2D
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Zero tilt image from one of the double tilt series through the Node of Ranvier
License
Attribution Only:
This image is licensed under a Creative Commons Attribution License.
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CCDB:50
*
Cite
Project:
P1136
Project name
Dynamics of membrane organization at the node of Ranvier
Description
Serial tomogram of a conventionally prepared peripheral nerve Node of Ranvier
Funding agency
NIH NINDS
Leader(s)
Mark Ellisman
Gina Sosinsky
Collaborator(s)
N.A
Start date
08-01-1995
End date
08-01-1995
Experiment
Experiment ID
30
Experiment date
10-04-2000
Title
Higher resolution tomogram of paranodal area, particularly septate junction
Purpose
Structure, localization of components
Experimenter(s)
Gina Sosinsky
Tom Deerinck
Microscopy product
Microscopy product ID
50
Instrument
JEOL 4000
Microscopy type
IVEM
Product type
double tilt
Image basename
node2X
Spatial Axis
Image Size
Pixel Size
X
800px
50 nm/pixel
Y
1000px
50 nm/pixel
Subject
Species
mouse
Scientific name
mus musculus
Strain
Unspecified
Age
3 days
Age class
neonate
Tissue section
Anatomical location
dorsal root
Microtome
Leica Ultracut UCT
Tissue product storage
Liquid nitrogen
Thickness
0.5 µm
Specimen description
System
peripheral nervous system
Structure
Node of Ranvier
Tissue
peripheral nerve
Cell type
Schwann cell
Imaging parameters
Type
Electron microscopy product
Magification
40000
Accelerating voltage
400 KeV
Imaging product type
Type
Double tilt
X min range
-60 degrees
X max range
60 degrees
X tilt increment
2 degrees
Y min range
-60 degrees
Y max range
60 degrees
Y tilt increment
2 degrees
×
Citation Information
Mark Ellisman, Gina Sosinsky, N.A (1995) CCDB:50, mus musculus, Node of Ranvier, Schwann cell. CIL. Dataset. https://doi.org/doi:10.7295/W9CCDB50