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Triple labeled confocal image of the cerebral cortex of a non-transgenic mouse, immunolabeled for mGluR5 (red), alpha synuclein (green) and counterstained with DAPI (blue) to reveal cell nuclei.
Full resolution image description
Zip archive containing the 3 channel image file in tiff format (112006cccc_RGB.tiff). Also included is the .oif header file generated by the Olympus Fluoview, which gives additional detail on microscope settings.
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Attribution Only: This image is licensed under a Creative Commons Attribution License. View License Deed | View Legal Code

CCDB:4055*  Cite 
Project: P1723
Project name
Localization of Metabotropic Glutamate Receptors in Alpha Synuclein Overexpressing Mouse
Description
Characterization of staining for mGluR5 glutamate receptor in animal model of Parkinsonian disorders
Funding agency
Branfman Family Foundation
Leader(s)
Diana Price
Collaborator(s)
Edward Rockenstein
Eliezer Masliah
Mark Ellisman
Start date
unspecified
End date
unspecified
 
Experiment
Experiment ID
3482
Title
Comparison of mGluR5 and synuclein staining
Purpose
To determine the relationship between mGluR5 and alpha synuclein staining in different lines of alpha synuclein overexpressing mouse
Experimenter(s)
Diana Price
Microscopy product
Microscopy product ID
4055
Instrument
Olympus Fluoview 1000
Microscopy type
laser scanning confocal
Product type
SURVEY
Image basename
112006cccc
Spatial Axis Image Size Pixel Size
X 1024px 0.207 um/pixels
Y 1024px 0.207 um/pixels
Subject
Species
mouse
Scientific name
mus musculus
Strain
C57BL/6-DBA/2
Group by
Genetic Modification
Treatment
None
Age class
adult
Tissue section
Microtome
Vibratome
Thickness
80 µm
Specimen description
Organ
brain
System
central nervous system
Imaging parameters
Type
Light microscopy product
Immersion medium
oil
Mounting medium
Prolong (Molecular Probes)
Lens
Olympus PlanApo 60X oil
Lens magnification
X
Numerical aperture
1.42
Notes
DAPI was added to the mounting medium
Imaging product type
Type
Optical section
Description
Only a single optical section was taken for each image.
Imaging product type
Type
Survey
Description
survey sections of different regions of brain