A slice by slice view from the backprojected volume of Caulobacter crescentus
Full resolution image description
A .tar file containing 2 MRC format backprojected volumes of Caulobacter crescentus. One is the uncropped version of the volume with XY slices flipped with XZ slices. The other is a smaller cropped and flipped version of the volume.
Both volumes have been binned by 2 from the original resolution
Downsampled image description
A single MRC file of the volume that has been cropped and flipped after backprojecting.
File format
MRC
Volume_dimension
1172, 2046, 184
Volume scale
0.001, 0.001, 0.0005
Animation description
A .mpg animation file of the slice by slice view of the backprojected volume.
The zero degree tilt view of the raw data acquired from an IVEM of a Caulobacter crescentus sample, strain Snc04
Full resolution image description
A .tar file containing all necessary IMOD processing files for a tomographic reconstruction of Caulobacter crescentus. Raw MRC tilt data is included in this
Animation description
A .mpg animation of the aligned tilt series acquired from an IVEM of a Caulobacter crescentus sample, strain Snc04
Produce a series of tomographic data of the normal morphology of Caulobacter Crescentus using rapid freezing methods.
Experimenter(s)
Patrick Viollier
Tom Deerinck
Microscopy product
Microscopy product ID
3998
Instrument
JEOL 4000 #1
Microscopy type
IVEM
Product type
SINGLE TILT
Image basename
cb012
Spatial Axis
Image Size
Pixel Size
X
4096px
0.5 nm/pixels
Y
4096px
0.5 nm/pixels
Subject
Species
Caulobacter
Scientific name
Caulobacter crescentus (Snc04)
Strain
Snc04
Treatment
None
Age class
N/A
Tissue section
Anatomical location
N/A
Tissue product storage
N/A
Thickness
0.5 µm
Specimen description
Cell type
Caulobacter crescentus
Imaging parameters
Type
Electron microscopy product
Magification
0
Accelerating voltage
400 keV
Specimen preparation
Protocol used
1) Induced for 1 hour at 30 degrees2) Labeled for 2 hours at 30 degrees (in presence of 1.8 micromolar ReAsH-EDT2)3) Post fixed with _0.01%_ Acrolein and 2.5% of Glut. in 0.01M Sodium Cocadalyte buffer.4) HPF5) FS by using Epoxy resin as fixative: 20% of the complete Araldite/Epon formulation in acetone. Infiltration and embedding in 100% of resin complex.6) 0.5um sections were cut and collected on gold grids. Sections were post stained with 4% of Osmium followed by Sato lead and finally 10nm was applied on both sides of sections.
Imaging product type
Type
Single tilt
Description
singlet_desc
Min range
-52 degrees
Max range
66 degrees
Tilt increment
2 degrees
Citation Information
Lucy Shapiro, Harley McAdams, Patrick H. Viollier, Tom Deerinck, Mason Mackey, John Crum, Mark Ellisman (2003) CCDB:3998, Caulobacter crescentus (Snc04), Caulobacter crescentus. CIL. Dataset. https://doi.org/doi:10.7295/W9CCDB3998
