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Reconstruction
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Display image description
A maximum intensity projection image of a caulobacter crescentus specimen taken on an IVEM.
Full resolution image description
A .tar file containing to .mrc format slice by slice volume reconstructions. One is a trimmed stack of the other larger volume. Both files were made using IMOD's tomographic reconstruction program.
Volume_dimension
1156, 3116, 1269
Animation description
A .mpg file movie of the slice by slice reconstructed volume of a caulobacter crescentus specimen.
Image 2D
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Display image description
A Caulobacter crescentus specimen taken at a 0 degree tilt. Gold fiducials are 10 and 15 nm in diameter.
Full resolution image description
A .tar file containing all processing files for an IMOD volume reconstruction. Along with necessary .st, .preali, .ali and .rawtlt files is a notes.txt file containing some information necessary for proper processing.
Animation description
a .mpg format movie file showing the aligned tilt series taken on an IVEM, shot onto film.
License
Attribution Only: This image is licensed under a Creative Commons Attribution License. View License Deed | View Legal Code

CCDB:3699*  Cite 
Project: P1220
Project name
Caulobacter
Description
Tomography of Caulobacter crescentus
Funding agency
Department of Energy
Leader(s)
Lucy Shapiro
Harley McAdams
Collaborator(s)
Patrick H. Viollier
Tom Deerinck
Mason Mackey
John Crum
Mark Ellisman
Start date
07-01-2003
End date
unspecified
 
Experiment
Experiment ID
3439
Experiment date
04-01-2007
Title
Tomographic reconstruction of caulobacter
Purpose
To produce a series of tomographic reconstructions of the normal morphology of caulobacter using traditional fixing methods.
Experimenter(s)
Tom Deerinck
Guido Gaietta
Microscopy product
Microscopy product ID
3699
Instrument
JEOL 4000 #1
Microscopy type
IVEM
Product type
SINGLE TILT
Image basename
cb0
Spatial Axis Image Size Pixel Size
X 4482px
Y 6678px
Subject
Species
Caulobacter crescentus
Scientific name
Caulobacter crescentus (Snc04)
Strain
Snc04
Age class
N/A
Tissue section
Anatomical location
N/A
Tissue product storage
N/A
Thickness
0.4 µm
Specimen description
Cell type
Caulobacter crescentus
Imaging parameters
Type
Electron microscopy product
Recording medium
film
Magification
30000
Specimen preparation
Protocol used
Induced for 1 hour at 30 degrees CLabeled in M2G medium for 2 hours at 30 degrees C (in the presence of 1.8 micromolar ReAsH-EDT2) and photoconvertedSamples were fixed with 2.5% glutaraldehyde with 1% acrolein in 0.05M sodium cacodylate pH 7.4 with 3 mM CaCl2 and 1 % sucrose followed by conventional osmium tetroxide/potassium ferrocyanide post-fixation, dehydration and embedding.
Imaging product type
Type
Single tilt
Description
singlet_desc
Min range
-60 degrees
Max range
60 degrees
Tilt increment
2 degrees
Notes
10 and 15 nm gold fiducials