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Reconstruction
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Display image description
An image from the slice by slice volume reconstruction of a Caulobacter crescentus specimen.
Full resolution image description
A .tar file containing a single volume reconstruction created using TxBR.
File format
MRC
Volume_dimension
1164, 3012, 206
Animation description
A .mpg animation file of a Caulobacter crescentus specimen. The animation shows a maximum intensity projection of the final reconstructed volume, rotated about the y-axis.
Image 2D
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Display image description
An image from the tilt series taken from the IVEM of a Caulobacter crescentus specimen. This image is taken at the zero degree tilt angle.
Full resolution image description
A .tar file containing all the files necessary for tomographic processing using TxBR quadratic reconstruction. The .preali file is the mode 6 rawdata in MRC format.
Animation description
A .mpg animation file showing the tilt series taken from the IVEM of a Caulobacter crescentus specimen.
License
Attribution Only: This image is licensed under a Creative Commons Attribution License. View License Deed | View Legal Code

CCDB:3647*  Cite 
Project: P1220
Project name
Caulobacter
Description
Tomography of Caulobacter crescentus
Funding agency
Department of Energy
Leader(s)
Lucy Shapiro
Harley McAdams
Collaborator(s)
Patrick H. Viollier
Tom Deerinck
Mason Mackey
John Crum
Mark Ellisman
Start date
07-01-2003
End date
unspecified
 
Experiment
Experiment ID
3374
Experiment date
05-12-2004
Title
Tomographic reconstruction of caulobacter
Purpose
Produce a series of tomographic data of the normal morphology of Caulobacter Crescentus using rapid freezing methods.
Experimenter(s)
Patrick Viollier
Tom Deerinck
Microscopy product
Microscopy product ID
3647
Instrument
JEOL 4000 #1
Microscopy type
IVEM
Product type
SINGLE TILT
Image basename
caulo4
Spatial Axis Image Size Pixel Size
X 2242px 1.42 nm/pixels
Y 3340px 1.42 nm/pixels
Subject
Species
Caulobacter
Scientific name
Caulobacter crescentus (NA1000)
Strain
NA1000
Treatment
None
Age class
N/A
Tissue section
Thickness
0.5 µm
Specimen description
Cell type
Caulobacter crescentus
Imaging parameters
Type
Electron microscopy product
Recording medium
film
Magification
25000
Accelerating voltage
400 kV
Imaging product type
Type
Single tilt
Description
singlet_desc
Min range
-60 degrees
Max range
60 degrees
Tilt increment
2 degrees
Notes
A tilt series of a Caulobacter Crescentus specimen taken from -60 to +60 degrees with 2 degree increments. No missing angles exist within this range. Shot by James Obayashi.