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Reconstruction
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Display image description
an image of slice 210 from the reconstructed volume.
Full resolution image description
one .mrc file of the final reconstructed volume using TxBR's quadratic transforms.
Volume_dimension
2242, 3340, 360
Volume scale
0.001, 0.001, 0.001
Animation description
a .mpg file of the reconstructed slice by slice volume.
Image 2D
Image Data Download Options...
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Display image description
0 degree view of the tilt series taken of a Caulobacter Crescentus specimen.
Full resolution image description
Included files are all used / produced by TxBR for the tomographic reconstruction process. In addition, the .preali mrc file in floating point (map mode 2) is included, as well as the .rawtlt file, .fid fiducials file and digital notes on the reconstruction process.
Animation description
a .mpg file showing the aligned tilt series stack of the Caulobacter Crescentus specimen
License
Attribution Only: This image is licensed under a Creative Commons Attribution License. View License Deed | View Legal Code

CCDB:3627*  Cite 
Project: P1220
Project name
Caulobacter
Description
Tomography of Caulobacter crescentus
Funding agency
Department of Energy
Leader(s)
Lucy Shapiro
Harley McAdams
Collaborator(s)
Patrick H. Viollier
Tom Deerinck
Mason Mackey
John Crum
Mark Ellisman
Start date
07-01-2003
End date
unspecified
 
Experiment
Experiment ID
3374
Experiment date
05-12-2004
Title
Tomographic reconstruction of caulobacter
Purpose
Produce a series of tomographic data of the normal morphology of Caulobacter Crescentus using rapid freezing methods.
Experimenter(s)
Patrick Viollier
Tom Deerinck
Microscopy product
Microscopy product ID
3627
Instrument
JEOL 4000
Microscopy type
IVEM
Product type
SINGLE TILT
Image basename
caulo1
Subject
Species
Caulobacter
Scientific name
Caulobacter crescentus
Strain
3724
Treatment
None
Age class
not applicable
Specimen description
Cell type
Caulobacter crescentus
Imaging parameters
Type
Electron microscopy product
Recording medium
film
Magification
30000
Accelerating voltage
400 keV
Notes
0.5 um thick sample
Specimen preparation
Protocol used
High Pressure Freezing (HPF): completed on January 26th, 2006 using Balzers HPM 010 in 100 micron deep gold plated copper planchettes. Bacteria was centrifuged decanted and paced applied to planchettes.Free Substitution: Started Friday January 26th.1. Cocktail - 2% Osmium tetroxide, 0.5% urynal acetate in anhydrous acetone2. Time -T1: -90 Celsius for 72 hoursS1: 2 Celsius per hour for 15 hoursT2: -60 Celsius per hour for 12 hoursS2: 5 Celsius per hour for 12 hoursT3: 0 Celsius HoldLeica FSU
Imaging product type
Type
Single tilt
Description
singlet_desc
Min range
-66 degrees
Max range
66 degrees
Tilt increment
2 degrees
Notes
Single tilt Caulobacter Crescentus series.