Caulobacter Crescentus specimen from the tilt series. This particular image was taken from the 0 degree tilt.
Full resolution image description
all IMOD processing files needed for a slice by slice volume reconstruction. Images, and consequently the original mrc .st file have not been binned and are their original 4k by 6k file sizes.
Animation description
a .mpg movie file of the tilt series taken of a caulobacter crescentus specimen. Movie starts at the zero degree tilt, and then rocks back and forth before moving back to the zero degree tilt.
Produce a series of tomographic data of the normal morphology of Caulobacter Crescentus using rapid freezing methods.
Experimenter(s)
Patrick Viollier
Tom Deerinck
Microscopy product
Microscopy product ID
3566
Instrument
JEOL 4000
Microscopy type
IVEM
Product type
SINGLE TILT
Image basename
p1220_b39_e5_2bin4
Spatial Axis
Image Size
Pixel Size
X
2242px
1 nm/pixels
Y
3340px
1 nm/pixels
Subject
Species
Caulobacter
Scientific name
Caulobacter crescentus
Strain
3724
Treatment
None
Age class
not applicable
Tissue section
Anatomical location
N/A
Thickness
0.5 µm
Specimen description
Cell type
Caulobacter crescentus
Imaging parameters
Type
Electron microscopy product
Recording medium
film
Magification
25000
Accelerating voltage
400 kV
Specimen preparation
Protocol used
1. Cocktail - 2% Osmium tetroxide, 0.5% urynal acetate in anhydrous acetone2. Time -T1: -90 Celsius for 72 hoursS1: 2 Celsius per hour for 15 hoursT2: -60 Celsius for 12 hoursS2: 5 Celsius per hour for 12 hoursT3: -20 Celsius HoldLeica FSU
Imaging product type
Type
Single tilt
Description
singlet_desc
Min range
-66 degrees
Max range
68 degrees
Tilt increment
2 degrees
Citation Information
Lucy Shapiro, Harley McAdams, Patrick H. Viollier, Tom Deerinck, Mason Mackey, John Crum, Mark Ellisman (2003) CCDB:3566, Caulobacter crescentus, Caulobacter crescentus. CIL. Dataset. https://doi.org/doi:10.7295/W9CCDB3566
