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Reconstruction
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Display image description
Caulobacter image from the reconstructed volume dataset
Full resolution image description
.tar file contains only the reconstructed volume file in .mrc format.
Volume_dimension
2242, 3340, 584
Volume scale
0.6, 0.6, 0.5
Animation description
a .mpg file of a reconstruction of a caulobacter crescentus specimen. Grayscale colors are inverted (to positive) from the thumbnail images.
Image 2D
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Display image description
Caulobacter Crescentus image taken from the tomographic data set at the 0 degree angle.
Full resolution image description
Raw image data of a caulobacter for project 1220, specimen number 3724. Includes 2 .mrc files (.ali and .preali), a .rawtlt file and a .fid file.
Animation description
a .mpg movie file of the aligned tilt series of a caulobacter crescentus specimen. Movie begins at the 0 degree orientation
License
Attribution Only: This image is licensed under a Creative Commons Attribution License. View License Deed | View Legal Code

CCDB:3548*  Cite 
Project: P1220
Project name
Caulobacter
Description
Tomography of Caulobacter crescentus
Funding agency
Department of Energy
Leader(s)
Lucy Shapiro
Harley McAdams
Collaborator(s)
Patrick H. Viollier
Tom Deerinck
Mason Mackey
John Crum
Mark Ellisman
Start date
07-01-2003
End date
unspecified
 
Experiment
Experiment ID
3374
Experiment date
05-12-2004
Title
Tomographic reconstruction of caulobacter
Purpose
Produce a series of tomographic data of the normal morphology of Caulobacter Crescentus using rapid freezing methods.
Experimenter(s)
Patrick Viollier
Tom Deerinck
Microscopy product
Microscopy product ID
3548
Instrument
JEOL 4000
Microscopy type
IVEM
Product type
SINGLE TILT
Image basename
p1220_3724e5_2
Spatial Axis Image Size Pixel Size
X 2242px 1 nm/pixels
Y 3340px 1 nm/pixels
Subject
Species
Caulobacter
Scientific name
Caulobacter crescentus
Strain
3724
Treatment
None
Age class
not applicable
Tissue section
Anatomical location
N/A
Thickness
0.5 µm
Specimen description
Cell type
Caulobacter crescentus
Imaging parameters
Type
Electron microscopy product
Recording medium
film
Magification
30000
Accelerating voltage
400 kV
Notes
Objective aperture at 2
Specimen preparation
Protocol used
1. Cocktail - 2% Osmium tetroxide, 0.5% urynal acetate in anhydrous acetone2. Time -T1: -90 Celsius for 72 hoursS1: 2 Celsius per hour for 15 hoursT2: -60 Celsius for 12 hoursS2: 5 Celsius per hour for 12 hoursT3: -20 Celsius HoldLeica FSU
Imaging product type
Type
Single tilt
Description
singlet_desc
Min range
-66 degrees
Max range
70 degrees
Tilt increment
2 degrees