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Reconstruction
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Display image description
montage of cerebellar cortex triple labeled for Hoescht stain (blue), IP3 receptor (green) and GFAP (red)
Full resolution image description
mosaic reconstructed with 10% overlap; image normalization performed using ImageJ
Volume_dimension
18701, 17360, 10
Volume scale
0.36, 0.36, 1
License
Attribution Only: This image is licensed under a Creative Commons Attribution License. View License Deed | View Legal Code

CCDB:23*  Cite 
Project: P1188
Project name
Brain Maps
Description
Creation of high resolution large scale brain maps of protein expression
Funding agency
NIH
Leader(s)
Tom Deerinck
Collaborator(s)
James Bouwer and Mark Ellisman and Maryann Martone
Start date
08-01-2002
End date
unspecified
 
Experiment
Experiment ID
15
Experiment date
06-20-2002
Title
High resolution brain maps
Purpose
high resolution protein labeling map of selected brain regions
Experimenter(s)
Tom Deerinck
Microscopy product
Microscopy product ID
23
Instrument
BioRad RTS 2000MP Multiphoton
Microscopy type
multiphoton
Product type
optical section series/mosaic
Image basename
071202tjd
Spatial Axis Image Size Pixel Size
X 512px 0.36 µm
Y 478px 0.36 µm
Subject
Species
rat
Scientific name
rattus norvegicus
Strain
Sprague Dawley
Treatment
none
Age
2 months
Age class
adult
Tissue section
Anatomical location
whole brain
Microtome
vibratome
Thickness
80 µm
Specimen description
Organ
brain
System
central nervous system
Map location
View location
Imaging parameters
Type
Light microscopy product
Immersion medium
oil
Mounting medium
gelvatol
Lens magnification
X
Numerical aperture
1.3
Imaging product type
Type
Optical section
Description
optical section mosaic
Cutting plane
transverse
Z resolution
1 um