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Maximum intensity projection through a computationally deblurred transmitted light series of a stained Purkinje cell dendrite from rat cerebellum. Through focus series was taken from a 2 um section prepared for electron microscopy
Full resolution image description
Zip file containing volume in Analyze 7.5 format, both .img and .hdr files
Volume_dimension
244, 564, 65
Segmentation
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Attribution Only: This image is licensed under a Creative Commons Attribution License. View License Deed | View Legal Code

CCDB:15*  Cite 
Project: P1119
Project name
Correlated Microscopy of Dendritic Spines
Description
Measurements of spine parameters using light microscopy and electron tomography
Funding agency
NIH
Leader(s)
Maryann Martone
Collaborator(s)
Naoko Yamada; Gordun Arbuthnott; Cali Ingham; Stephen Young
Start date
01-01-1992
End date
unspecified
 
Experiment
Experiment ID
10
Title
spiny dendrite
Purpose
how well dendritic spines can be detected and measured using LM
Experimenter(s)
Naoko Yamada
Microscopy product
Microscopy product ID
15
Instrument
BioRad MRC 1024 Confocal
Microscopy type
transmitted light
Product type
through focus series
Image basename
pccor10_dc
Spatial Axis Image Size Pixel Size
X 1024px
Y 1024px
Subject
Species
rat
Scientific name
rattus norvegicus
Strain
Sprague Dawley
Treatment
none
Age class
adult
Tissue section
Anatomical location
cerebellum
Microtome
ultramicrotome
Thickness
2 µm
Specimen description
Organ
brain
System
central nervous system
Structure
spiny dendrite
Cell type
Purkinje neuron
Imaging parameters
Type
Light microscopy product
Immersion medium
oil
Mounting medium
epoxy
Lens magnification
X
Numerical aperture
1.4
Imaging product type
Type
Through focus
Description
see image map
Z step
.1 um