Tomographic reconstruction of stained Purkinje cell dendrite from rat cerebellum. Maximum intensity
projection through the volume, with constrast reversed so that dendrite appears bright against background
Full resolution image description
Pccor10_inv is an unprocessed volume with the contrast reversed (spiny dendrite appears bright)
Zero tilt image from a single tilt series through a 2 um thick section of a Purkinje cell dendrite that has been intracellularly injected and photooxidized
Full resolution image description
This file contains the original tilt images; pccor10.????.f.Z; compressed.To uncompress use the uncompress command in Unix; Also contains the aligned and cropped tilt images; pccor10.????.crop.The file containing the fiducial marks for the alignment
manual segmentation using Xvoxtrace; surfaced with Synu using Nuage algorithm; tarfile contains all synu objects listed; the associated Viewdata file required by Synu for viewing; and Xvoxtrace file (pccor10mm.trace) used to contour the objects.
Segmentation file description
Tar file containing original trace file (MM.trace) with manual contours produced with Xvoxtrace and the surface renderings of the dendritic shaft (shaft.synu) and the individual dendritic spines (sp*.synu) in Synu format, along with the Viewdata file required for viewing with Synuview.
