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Reconstruction
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Display image description
Maximum intensity projection through an optical section series of an intracellularly injected basket cell from the rat cerebellar vermis.
Full resolution image description
Zipped archive containing the optical section series in Biorad PIC format
Volume_dimension
1024, 1024, 36
Volume scale
0.237, 0.237, 0.3
Animation description
Rotation loop of maximum intensity projection through a filled basket cell from the rat cerebellar vermis. The movie was created in Imaris and shows an orientation grid superimposed.
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Attribution Only: This image is licensed under a Creative Commons Attribution License. View License Deed | View Legal Code

CCDB:1132*  Cite 
Project: P1123
Project name
CCDB rat test data
Description
Confocal images
Funding agency
NIH
Leader(s)
Maryann Martone
Collaborator(s)
Diana Price and Andrea Thor
Start date
10-23-2001
End date
unspecified
 
Experiment
Experiment ID
4
Experiment date
02-27-2002
Title
Intracellular injection of hippocampal astrocyte
Purpose
to obtain multi resolution data for CCDB
Experimenter(s)
Diana Price & Andrea Thor
Microscopy product
Microscopy product ID
1132
Instrument
Biorad MRC 1024 confocal
Microscopy type
laser scanning confocal
Product type
OPTICAL SECTION
Image basename
PC5AZ
Spatial Axis Image Size Pixel Size
X 1024px 0.237 um/pixels
Y 1024px 0.237 um/pixels
Subject
Species
rat
Scientific name
rattus norvegicus
Strain
Sprague Dawley
Age class
adult
Tissue section
Anatomical location
cerebellum
Microtome
Vibratome
Specimen description
Organ
brain
System
central nervous system
Cell type
cerebellar basket cell
Imaging parameters
Type
Light microscopy product
Immersion medium
oil
Mounting medium
gelvatol
Lens magnification
X
Numerical aperture
1.4
Notes
mmartone
Imaging product type
Type
Optical section
Z resolution
0.3 um
Notes
Z resolution determined from optical section header file; was not verified