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Optical section series through a protoplasmic astrocyte in rat hippocampal area CA1 intracellulaly injected Lucifer Yellow and imaged with confocal microscopy

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CCDB:1061*  Cite 
Project: P1230
Project name
Astrocyte Development
Description
Postnatal development of protoplasmic astrocytes
Funding agency
NIH
Leader(s)
Eric Bushong
Collaborator(s)
Maryann Martone
Mark Ellisman
Start date
02-01-2002
End date
unspecified
 
Experiment
Experiment ID
23
Title
Morphology of astrocytes in 4 week old hippocampus
Purpose
Examine the morphology of 4 week old astrocytes
Experimenter(s)
Eric Bushong
Microscopy product
Microscopy product ID
1061
Instrument
Biorad Radiance2000
Microscopy type
single photon confocal
Product type
optical section series
Image basename
4wk-ly14
Spatial Axis Image Size Pixel Size
X 1024px 0.072549 µm
Y 1024px 0.072549 µm
Subject
Species
rat
Scientific name
rattus norvegicus
Strain
Sprague Dawley
Group by
NA
Treatment
NA
Age
4 weeks
Age class
young adult
Tissue section
Anatomical location
hippocampus
Microtome
vibratome
Tissue product storage
coverslipped
Thickness
100 µm
Specimen description
Organ
brain
System
central nervous system
Cell type
protoplasmic astrocyte
Imaging parameters
Type
Light microscopy product
Immersion medium
oil
Mounting medium
gelvatol
Lens
Nikon
Lens magnification
X
Numerical aperture
1.4
Refractive index
1.5
Imaging product type
Type
Optical section
Z resolution
0.25 um