Licensing

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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:9693^* Cite

Description

Overview of Coleps hirtus cell seen in this median transverse section. This barrel-shaped cell has a few rows of cilia and the cell is covered with calcified polysaccharide platelets that are housed within the alveoli. In this TEM the platelets are absent as they dissolved during preparation for electron microscopy. The macronucleus, a food vacuole, and a large empty vacuole is evident. Typical ciliate cytoplasm is present. TEM taken on 5/3/69 by R. Allen with Philips 300 operating at 60kV. Neg. 3,120X. Bar = 2µm.

Technical Details

A print of the negative was scanned and processed in Photoshop. This image is best used for qualitative analysis. A high resolution image is in the library at (CIL:38828). Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. Additional information is available at (http://www5.pbrc.hawaii.edu/allen/).

Biological Sources

NCBI Organism Classification: Coleps hirtus
Cell Type: eukaryotic cell; Eukaryotic Protist; Ciliated Protist
Cell Line: Cambridge Culture Collection, Cambridge, England, catalog # LB1656/1
Cellular Component: cellular_component; plasma membrane; alveolar membranes; contractile vacuole; macronucleus; kinetal rows of cilia

Biological Context

Biological Process: ciliary or flagellar motility; detection of calcium ion
Molecular Function: calcium deposition

Attribution

Names: Richard Allen

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL9693
Archival Resource Key (ARK): ark:/b7295/w9cil9693

Grouping This image is part of a group.

Imaging

Image Type: transmission electron microscopy (TEM); illumination by electrons
Image Mode: detection of electrons; film
Parameters Imaged: electron density
Source of Contrast: stain with broad specificity
Visualization Methods: stain with broad specificity; osmium tetroxide; uranyl salt; lead salt
Processing History: film; Print from negative scanned for Photoshop.
Data Qualifiers: processed data; suitable for spatial measurements

Sample Preparation

Methods: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment
Relation To Intact Cell: microtome-sectioned tissue

Dimensions

Spatial Axis	Image Size	Pixel Size
X	3318px	——
Y	3374px	——