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Licensing
Public Domain: This image is in the public domain and thus free of any copyright restrictions. However, as is the norm in scientific publishing and as a matter of courtesy, any user should credit the content provider for any public or private use of this image whenever possible. Learn more
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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:9685*  Cite 
Description

Cross section of a Coleps cilium showing the axoneme consisting of 9 doublet and 2 central microtubules. Doublets have a complete A subfiber and an attached B subfiber. Two dynein arms, outer and inner, extend from the A subfiber. The ciliary membrane with a dense external coat surrounds the ciliary axoneme. The plasma membrane has a glycocalyx composed of the dense coat plus longer filaments. Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. TEM taken on 5/28/69 by R. Allen with Philips 300 operating at 60kV. Neg. 92,500X. The negative was printed to paper and the image was scanned with a flatbed scanner and 100nm bar added in Photoshop. This digitized image is available for qualitative analysis. A raw, unprocessed, high resolution version of this image (CIL:2871) is in the library and available for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).

Biological Sources
NCBI Organism Classification
Coleps hirtus
Cell Type
eukaryotic cell
Eukaryotic Protist
Ciliated Protist
Cell Line
Cambridge Culture Collection, Cambridge, England, catalog # LB1656/1
Cellular Component
cilium axoneme
axonemal microtubule
axonemal dynein complex
glycocalyx
Attribution
Names
Richard Allen
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL9685
Archival Resource Key (ARK)
ark:/b7295/w9cil9685
Grouping This image is part of a group.
Imaging
Image Type
transmission electron microscopy (TEM)
illumination by electrons
Image Mode
detection of electrons
film
Parameters Imaged
electron density
Source of Contrast
stain with broad specificity
Visualization Methods
stain with broad specificity
osmium tetroxide
uranyl salt
lead salt
Processing History
stain with broad specificity
recorded image
film
Print from negative scanned to Photoshop.
Data Qualifiers
processed data
suitable for spatial measurements
Dimensions
Spatial Axis Image Size Pixel Size
X 3000px ——
Y 2364px ——